Product Class: Other

Pyrophosphatase, Inorganic (E. coli)
Gray property icon for recombinant Gray property icon for heat inactivation no

GMP-grade reagent also available. Learn more.

Product Introduction

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.


Catalog # Size Concentration
M0361S 10 units 100 units/ml
M0361L 50 units 100 units/ml

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Product Information

Description

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:

Product Source

PPase is prepared from an E. coli strain containing an engineered His-tag E. coli inorganic pyrophophatase gene.
This product is related to the following categories:
Phosphatases,
RNA Modification,
RNA Synthesis In vitro Transcription (IVT),
Nucleotide Solutions for RNA,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2 and 2 mM  PPi).

Storage Buffer

20 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

No

Application Features

  • Increasing RNA yield in transcription reaction; enhancing DNA replication.

Product Notes

  1. Use 1–3 units per ml in a high yield in vitro RNA synthesis reaction.
  2. While a specific NEBuffer is not supplied, E. coli Inorganic Pyrophosphatase works well in any buffer containing Mg++ (1-10 mM). This enzyme is typically used in RNA IVT reactions, where the Mg++ concentration falls within this range.

Protocols, Manuals & Usage

Application Notes

FAQs & Troubleshooting

Tech Tips

The enzyme works well in most reaction buffers containing Mg2+.
It cannot be inactivated by heating at 70°C.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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