The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0356S     -20       RNA 5' Pyrophosphohydrolase (RppH) M0356SVIAL -20 1 x 0.04 ml 5,000 units/ml   NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X

Materials Sold Separately

• NEBuffer 2

1. Since RppH is active in the presence of Mg²⁺, it can be incubated with RNA Ligase or XRN-1.

1. Deana, A. et al. (2008). Nature. 451, 355-358.
2. Bessman, M.J. et al. (2001). JBC. 276, 37834.
3. Neri F, Rapelli S, Krepelova A, Incarnato D, Parlato C, Basile G, et al. (2017). Intragenic DNA Methylation prevents spurious transcription initiation. Nature. 543, 72–7.
4. Hetzel, Jonathan et al. (2016). Nascent RNA Sequencing Reveals Distinct Features in Plant Transcription. Proceeding of the National Academy of Sciences. 113.43, 12316–12321.

1. Removal of pyrophosphate from 5 triphosphorylated RNA using RppH (NEB #M0356)
2. Decapping eukaryotic mRNA with RppH (NEB #M0356)
3. Cappable-seq for prokaryotic transcription start site (TSS) determination
4. One-pot digestion of 5´ triphosphorylated RNA using RppH (NEB #M0356) & XRN-1 (NEB #M0338)
   
   
   

1. Can RppH replace Tobacco Acid Pyrophosphatase (TAP) as a decapping enzyme?
2. Can RppH be heat inactivated?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0356S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0356S_v1_10102208
• M0356S_v1_10170000

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.