hSMUG1
Product information
hSMUG1
The large size of this product has been discontinued.
- Human single-stranded selective monofunctional uracil DNA glycosylase
- Monofunctional DNA glycosylase that catalyzes the hydrolysis of the N-glycosidic bond to release uracil and uracil-derivatives from DNA
- Major substrates include uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil
- Active on ss and dsDNA
Featured Video
-
Product Information
Highlights
Product Source
An E. coli strain which carries the cloned human SMUG1 gene.- This product is related to the following categories:
- DNA Repair Enzymes and Structure-specific Endonucleases
-
Reagents Supplied
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration -
M0336S -20 hSMUG1 M0336SVIAL -20 1 x 0.1 ml 5,000 units/ml NEBuffer™ 1 B7001SVIAL -20 1 x 1.25 ml 10 X Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
-
-
Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to excizse 1 pmol of deoxyuracil from a 34 mer oligonucleotide duplex containing a single dU site in a total reaction volume of 10 µl in 1 hour at 37°C.Reaction Conditions
1X NEBuffer™ 1
Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade
Incubate at 37°C1X NEBuffer™ 1
10 mM Bis-Tris-Propane-HCl
10 mM MgCl2
1 mM DTT
(pH 7 @ 25°C)Storage Buffer
10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°CHeat Inactivation
65°C for 20 minutesMolecular Weight
Theoretical: 29861 daltons
Unit Assay Conditions
1X NEBuffer 1 supplemented with 100 µg/ml Recombinant Albumin containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 µl. After release of dU, the oligonucleotide is cleaved by treating the resulting AP site with 100 mM NaOH for 10 minutes at 80°C. -
Related Products
Materials Sold Separately
-
Product Notes
- hSMUG1 has 50% activity on 5-hydroxymethyluracil when compared to uracil. hSMUG1 has 50% activity on ssDNA compared to ds DNA.
-
References
- Cannon-Crlson, S.V. et al. (1989). J. Biol. Chem. 264, 13306-13312.
- Masaoka, A. et al. (2003). Biochemistry. 42, 5003-5012.
- Wibley, J.E.A. et al. (2003). Cell. 11, 1647-1659.
- Singh, N. et al. (1998). Experimental Cell Research. 175, 184-191.
- Collins, A. et al. (1993). Carcinogenesis . 14, 1733-1735..
- Collins, A. et al. (1996). Environmental Health Perspectives. 104, 465-469.
-
Protocols, Manuals & Usage
-
Usage Guidelines
-
-
Tools & Resources
-
FAQs & Troubleshooting
-
Citations & Technical Literature
-
Citations
Product Citation Tool
-
-
Quality, Safety & Legal
-
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here. -
Specifications & Change Notifications
Recombinant Albumin (rAlbumin) replaces BSA beginning with Lot #10112346.The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] -
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number] -
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. -
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
-
Featured Videos
Other Products You May Be Interested In
No supporting documents available
This product has no supporting documents available for download. If you feel like supporting documents should be available for this product, please contact us.