Product Class: Other

Amylose Resin High Flow

Product Introduction

Amylose Resin High Flow is a cross-linked affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). This rigid matrix can be used in automated chromatography systems.

  • This resin is specially designed and cross-linked for use in automated chromatography systems
  • Highly specific binding of protein of interest fused to MBP allows for one-step purification
  • MBP is easily removed from the protein of interest by Factor Xa Protease
  • Binding capacity of >4mg MBP5*-paramyosin ΔSal fusion protein/ml amylose resin high flow
  • Can be used multiple times and can be regenerated

Catalog # Size Concentration
E8022S 15 ml
E8022L 100 ml

Product Information

Description

Amylose Resin High Flow is a cross-linked affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). This rigid matrix can be used in automated chromatography systems.

DNASU is a central repository for plasmid clones and collections that may also be helpful.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact the Customized Solutions team for further information.

This product is related to the following categories:
Amylose Purification (MBP-tag),
Affinity Purification,
Protein Purification,
This product can be used in the following applications:
MBP Affinity Tag,
Affinity Purification & Expression Tags,
Protein Purification,
Phage Display

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

20% Ethanol

Binding Capacity

>4 mg MBP5* -paramyosin δSal fusion protein / ml amylose high flow resin.

Can be Regenerated

Yes

Product Notes

  1. Store at 4°C. After use, resin should be stored in column buffer plus 0.02% sodium azide or 20% ethanol.
  2. Amylose Resin column should be washed with 5 volumes of column buffer before each use. 
    For optimum performance, load crude extract at < 60 cm/hour.
  3. When regenerating the column at 4°C, please note that 0.1% SDS can precipitate at that temperature. It is therefore recommended that the SDS solution be stored at room temperature until needed. The resin may be generated up to five times. 
  4. For a complete affinity purification protocol, download the pMAL Protein Fusion and Purification System technical bulletin (NEB #E8200) from www.neb.com.
  5. Regeneration: The packed resin may be regenerated by the following wash sequence: Water 3 - column volumes, 0.1% SDS - 3 column volumes, Water - 1 column volume, Column Buffer - 5 column volumes.

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What gravity column do you recommend?
  2. Much of my fusion protein flows through the amylose column. Is there anything I can do to improve my fusion’s affinity for the amylose column?
  3. How many times can I use the amylose column?
  4. What is known about binding in the presence of nonionic detergents?
  5. Can I substitute a different buffer and/or salt concentration in the Column Buffer?
  6. I see my intact fusion protein by SDS-PAGE when I run cells boiled in Sample Buffer, but when I check the crude extract the fusion is degraded.
  7. When I run my purified fusion protein on SDS-PAGE, why do I see multiple bands instead of a single band of the expected MW?
  8. Can I perform a batch purification using the amylose resin?
  9. Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
  10. Is the amylose resin damaged by storage at -20°C?
  11. What is the recommended flow rate and maximum pressure for Amylose Resin High Flow once packed into a column?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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