Luna® Cell Ready Lysis Module
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
E3032S |
Luna Cell Ready Lysis Module |
100 rxns | - | Unavailable in your region |
Luna® Cell Ready Lysis Module
Catalog #E3032
Product Introduction
- Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
- Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
- Minimal sample loss compared to alternative RNA extraction methods, especially at low cell input
- Compatible with high-throughput applications: cell lysis can take place at room temperature
- Optimal performance when paired with Luna Universal One-Step RT-qPCR kits: NEB #E3005 (dye-based) or NEB #E3006 (probe-based)
- Complete lysis and one-step RT-qPCR workflow available: NEB #E3030 (dye-based) or NEB #E3031 (probe-based)
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps. The module coordinates cell lysis, RNA release, and genomic DNA removal simultaneously, resulting in cell lysate ready for direct RT-qPCR analysis with Luna Universal One-Step RT-qPCR kits. Similar to other Luna products, the lysis buffer includes an inert blue tracking dye for visual assistance throughout the workflow.
This table lists cell lines that are compatible with the Luna Cell Ready Lysis Module. It includes results seen using a 5-log dilution series (100,000 - 10 cells per 50 µl lysis) and subsequent detection of 1 µl of lysate in a 20 µl one-step RT-qPCR reaction. For each cell line, linear results are indicated in the final column. In addition, some insect cell lines have been tested and are compatible.
Table 1: Validated Cell Lines
Cell line | Property | Species | Cell number per 50 μl lysate |
---|---|---|---|
A549 | Adherent | H. sapiens, Lung, carcinoma | 10 - 100,000 |
HEK293 | Adherent | H. sapiens, Kidney | 10 - 100,000 |
HeLa | Adherent | H. sapiens, Cervix, adenocarcinoma | 10 - 100,000 |
HepG2 | Adherent | H. sapiens, Liver, carcinoma | 10 - 1,000 |
NCI-H460 | Adherent | H. sapiens, Lung, carcinoma | 10 - 100,000 |
SK-N-SH | Adherent | H. sapiens, Brain, Neuroblastoma | 10 - 10,000 |
U2Os | Adherent | H. sapiens, Bone, osteosarcoma | 10 - 100,000 |
Jurkat | Suspension | H. sapiens, T lymphocyte, leukemia | 10 - 100,000 |
K-562 | Suspension | H. sapiens, Lymphoblast, leukemia | 10 - 100,000 |
- This product is related to the following categories:
- Luna® qPCR & RT-qPCR Products,
- PCR, qPCR & Amplification Technologies Products,
- This product can be used in the following applications:
- qPCR & RT-qPCR,
- DNA Amplification, PCR & qPCR
Kit Components
Kit Components
The following reagents are supplied with this product:
Properties & Usage
Materials Required but not Supplied
- Phosphate-buffered saline (PBS)
- Cells
- Eppendorf tubes
- PCR strip tubes
- PCR plates
- Pipettors and pipette tips (to minimize cross contamination, filter tips should be used)
Protocols, Manuals & Usage
Protocols
Manuals
Application Notes
Tools & Resources
Web Tools
FAQs & Troubleshooting
FAQs
- What cell lines are compatible with the Luna Cell Ready Lysis Module (NEB #E3032)?
- How many cells should I use for reliable detection?
- How many cells should I seed into cell culture plates to achieve optimal detection?
- Can cryopreserved cell lines or pelleted cells stored at -80°C be used with this kit?
- How can lysis efficiency be determined?
- Why is it important to evaluate RNA damage or inhibition during cell lysis and one-step RT-qPCR detection?
- What is the “Luna Cell Ready RNA Protection Reagent” and when should it be used?
- Should I include a No-RT control reaction?
- Will the blue tracking dye interfere with detection?
- Is cell washing prior to lysis necessary?
- Which lysis format should I use?
- What protocol should be used for cell lysis? Can cells be lysed at room temperature or using a short protocol?
- Can I use the cell lysates generated from the Luna Cell Ready Lysis Module for two-step RT-qPCR?
- When using cell lysates generated from Luna Cell Ready Lysis Module, can RT-qPCR experiments be set up at room temperature?
- How much cell lysate should I use as input? (NEB #3030)
- Why does the Luna Cell Ready One-Step RT-qPCR Kit contain more RT-qPCR reactions than lysis reactions?
- If either the Luna Cell Ready Lysis Module or Luna Universal One-Step RT-qPCR Kit runs out, can I order them separately?
- How can I obtain more technical help related to Luna Universal One-Step RT-qPCR Kit?
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- E3032S_v0_10058891
- E3032S_v1_10071649
- E3032S_v1_10073107
- E3032S_v1_10073108
- E3032S_v1_10073764
- E3032S_v1_10074942
- E3032S_v1_10107170
- E3032S_v1_10110340
- E3032S_v1_10116317
- E3032S_v1_10122375
- E3032S_v1_10131727
- E3032S_v1_10131887
- E3032S_v1_10135890
- E3032S_v1_10137148
- E3032S_v1_10140420
- E3032S_v1_10145286
- E3032S_v1_10150582
- E3032S_v1_10157247
- E3032S_v1_10160452
- E3032S_v1_10164428
- E3032S_v1_10166982
- E3032S_v1_10169739
- E3032S_v1_10174599
- E3032S_v1_10177191
- E3032S_v1_10181645
- E3032S_v1_10187451
- E3032S_v1_10194838
- E3032S_v1_10204102
- E3032S_v1_10208706
- E3032S_v1_10215724
- E3032S_v1_10232642
- E3032S_v1_10237007
- E3032S_v1_10242957
- E3032S_v1_10245850
- E3032S_v1_10250240
- E3032S_v1_10256906
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.Luna Cell Ready Lysis Buffer
Luna Cell Ready RNA Protection Reagent
Luna Cell Ready Protease
Luna Cell Ready Stop Solution
DNase I (RNase-free)
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us atgbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications
The supporting documents available for this product can be downloaded below.