Product Class: Other

NEB® 10-beta/Stable Outgrowth Medium

Product Introduction

  • Outgrowth Medium recommended for use with NEB 10-beta or NEB Stable Competent E. coli 
  • Use to dilute the outgrowth prior to plating
Catalog # Size Concentration
B9035S 100.0 ml

Product Information


Add NEB 10-beta/Stable Outgrowth Medium to freshly transformed NEB 10-beta or NEB Stable E. coli cells and incubate for up to 1 hour before plating on selective media. This medium can also be used to dilute the outgrowth prior to plating.
This product is related to the following categories:
Competent Cells Buffers & Diluents,
Buffers Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Product Notes

  1. NEB 10-beta/Stable Outgrowth Medium is shipped at 0°C or -80°C. Upon receipt, store at room temperature or 4°C.

FAQs & Troubleshooting


  1. What competent E. coli strains should I use NEB 10-beta/Stable Outgrowth Medium with?
  2. Can I use NEB 10-beta/Stable Outgrowth Medium with strains other than NEB 10-beta or NEB Stable Competent E.coli?
  3. When working with NEB 10-beta Competent E.coli and NEB Stable competent E.coli, do I need to perform outgrowth dilutions prior to plating with NEB 10-beta/Stable Outgrowth Medium?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective May 2, 2022, a secondary bottle supplier has been qualified. The alternative 30 mL bottle has a different cap color and may be used for relevant pack sizes with no change in product performance or stability.


The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.