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Validating an antibody for immunohistochemistry (IHC) use involves more than just observing a signal in cells. Cell Signaling Technology (CST) thoroughly tests the specificity of all antibodies recommended for IHC, and establishes optimal assay conditions to help you achieve the best results right from the start.
IHC-validated XP rabbit monoclonal antibodies from CST demonstrate the exceptional specificity and sensitivity required for the most accurate results in IHC and many other research applications. XP ‘eXceptional Perfomance’ monoclonal antibodies directed against novel targets provide new insights for basic and clinical researchers and scientists developing new drugs.
Phospho-Akt (Ser473) (D9E) Antibody Comparison |
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Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 was compared to three competitors’ IHC-approved Phospho-Akt (Ser473) antibodies. (A) The optimal dilution of each antibody was individually evaluated to maximize specific signal in untreated LNCaP cells (left) and minimize non-specific signal in LNCaP cells treated with LY294002 (PI3 Kinase Inhibitor) #9901 (right). (B) The determined optimal dilution for each antibody was used in IHC analysis of paraffin-embedded human breast carcinoma. Note stronger staining obtained with the XP® monoclonal antibody. |
Cat# | Name | Application | Species |
4060 | Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb | W IP IHC-P IHC-F IF-IC F | H M R Hm Mk Dm Z B (C) (X) (Dg) (Pg) |
8768 | Stat3α (D1A5) XP® Rabbit mAb | W IP IHC-P IF-IC ChIP | H M R Hm Mk (Pg) |
3777 | Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb | W IHC-P IF-IC F | H M R Mk |
4267 | W IP IHC-P IF-IC F | H M Mk | |
4290 | Her2/ErbB2 (D8F12) XP® Rabbit mAb | W IHC-P | H (M) (R) |
4370 | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb | W IP IHC-P IF-IC F | H M R Hm Mk Mi Dm Z B Dg Pg Sc (C) (Ce) |
3077 | Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb | W IP IHC-P IHC-F IF-IC F | H M R |
9188 | PTEN (D4.3) XP® Rabbit mAb | W IP IHC-P | H M R Mk Dg (C) |
12708 | HER3/ErbB3 (D22C5) XP® Rabbit mAb |
W IP IHC-P IF-IC F |
H (M) (R) (Mk) |