Product Class: Kit

Luna® Probe One-Step RT-qPCR Kit (No ROX) 


Catalog #E3007

Product Introduction

Rapid, sensitive and precise probe-based qPCR detection and quantitation of target RNA targets. 

Make a simpler choice

  • For instruments that do not require the ROX passive reference dye
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase

  • Novel, thermostable reverse transcriptase (RT) improves performance 
  • WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness

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Protocols, Manuals & Usage

Protocols

  1. Luna® Probe One-Step RT-qPCR Kit (No ROX) Protocol (NEB# E3007)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Application Notes

FAQs & Troubleshooting

FAQs

  1. How do I use qPCR to determine the concentration of my material?
  2. Can I set up my Luna® RT-qPCR at room temperature?
  3. What is the difference between probe- and dye-based versions of the Luna® qPCR Mixes?
  4. Should I use probe- or dye-based detection for my qPCR assays?
  5. How should I design primers for Luna® qPCR?
  6. How long should my amplicon be for qPCR?
  7. Why is the Luna® qPCR Mix blue? Will this dye interfere with detection?
  8. Can I use fast instrument settings with the Luna® qPCR Mix?
  9. Do I need to add ROX?
  10. How many dilutions should I use to make a standard curve?
  11. Why does NEB recommend 40-45 cycles?
  12. Does the Luna® qPCR Mix contain dUTP? Can I use carryover contamination prevention methods?
  13. Can I run multiplex reactions with the Luna® Probe One-Step RT-qPCR Kits? Do I need to change my reaction conditions?
  14. Can I use a ROX-labeled probe with the Luna® Probe Mixes that contain a universal ROX reference dye?
  15. Can alternative probe based detection strategies be used with the Luna® Probe Mix?
  16. How much primer and probe should I use with the Luna® Universal Probe RT-qPCR Kit?
  17. How do I choose between one-step RT-qPCR and two-step RT-qPCR?
  18. What RNA samples can be used in RT-qPCR with the Luna® Mix?
  19. How much RNA template should I use in my RT-qPCR reaction?
  20. Can I use longer targets in one-step RT-qPCR?
  21. What temperature should I use for cDNA synthesis with Luna® RT-qPCR kits?
  22. Should I include a no Luna® WarmStart® Enyzme Mix control (-RT Control)?
  23. Is the Monarch® Total RNA Miniprep Kit compatible with Luna® RT-qPCR Reagents?
  24. Are the Monarch RNA Cleanup Kits compatible with Luna RT-qPCR reagents?
  25. Can I use shorter cycling times?
  26. What are the differences between the Luna One-Step RT-qPCR products (NEB #E3006, #E3007, #M3019, #M3029 and #L4001)?
  27. Can I run the Luna® qPCR Mix on my qPCR instrument?
  28. Can I use the Luna Probe RT-qPCR mixes for viral detection?

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