Product Class: Kit

LunaScript® RT SuperMix Kit

LunaScript RT SuperMix Kit (NEB #E3010) includes supermix, No-RT control mix, and nuclease-free water.

Supermix only option now available, please see LunaScript RT SuperMix (NEB #M3010).

Featured in the ARTIC SARS-CoV-2 sequencing workflow (1) and a component in the NEBNext® ARTIC SARS-CoV-2 kits.

Product Introduction

  • Single-tube supermix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna® Reverse Transcriptase
  • Less than 15 minute first-strand cDNA synthesis protocol
  • Novel, thermostable Luna Reverse Transcriptase improves performance 
  • Combine with Luna qPCR master mixes (NEB #M3003, M3004) for robust RT-qPCR results
  • Eliminate pipetting errors with non-interfering, visible tracking dye
  • Primer-free version (NEB #E3025) available
  • Does include nuclease-free water and No-RT Control Mix

1. Josh Quick 2020. nCoV-2019 sequencing protocol v3 (LoCost). protocols.io 

Catalog # Size Concentration
E3010S 25.0 reactions
E3010L 100.0 reactions

Featured Videos

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  • RNase_Contamination_Anim_720x405

    Avoiding RNase Contamination

  • overview of qPCR

    Overview of qPCR

  • Luna Dots In Boxes

Protocols, Manuals & Usage

Protocols

  1. Protocol for LunaScript®  RT SuperMix Kit (E3010)
  2. Protocol for Two-step RT-qPCR using the LunaScript® RT SuperMix Kit (NEB #E3010) and the Luna® Universal qPCR Master Mix (NEB #M3003) or Luna Universal Probe qPCR Master Mix (NEB #M3004) 

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Application Notes

FAQs & Troubleshooting

FAQs

  1. Can I use  RNA samples purified from different storage conditions such as RNALater in my LunaScript®  cDNA synthesis reactions?
  2. Can I add gene-specific primers in my LunaScript®  cDNA synthesis reaction?
  3. Can I set up my cDNA synthesis reaction at room temperature when using the LunaScript®  RT SuperMix?
  4. Can I use a shorter incubation time for my cDNA synthesis reaction when using the LunaScript®  RT SuperMix?
  5. Can I use LunaScript® cDNA products directly in qPCR? How much can I use for detection in a qPCR reaction?
  6. How much RNA template should I use in my LunaScript®  cDNA synthesis reactions?
  7. How should I store LunaScript®  cDNA products?
  8. How should I choose between a one-step RT-qPCR or two-step RT-qPCR approach?
  9. Should I include a No-Reverse Transcriptase (RT) control reaction when setting up my cDNA synthesis reaction?
  10. What qPCR reagents do you recommend for detection of cDNA products?
  11. What temperature should I use for my LunaScript®  cDNA synthesis reaction?
  12. Why do I have low cDNA yields?
  13. Will the blue dye in the LunaScript®  RT SuperMix interfere with detection?
  14. How do I choose between the first strand cDNA synthesis kits from NEB?
  15. When using LunaScript cDNA products in downstream PCR (i.e., two-step RT-PCR), what volumes and PCR products can be used? 
  16. Can I use LunaScript RT SuperMix in RNA-seq workflows?
  17. How do I choose between LunaScript® RT SuperMix Kit and ProtoScript first strand cDNA synthesis kits?
  18. Can I use a shorter incubation time for my cDNA synthesis reaction when using the LunaScript® RT Master Mix (Primer-free)? Does longer incubation increase the yield of cDNA?
  19. How do I choose between Induro Reverse Transcriptase and the different LunaScript and ProtoScript products?
  20. How do I know whether my template RNA is of good quality?

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